Porins let drugs in, efflux pumps kick them out β alone, neither does much damage. Real resistance in Gram-negatives needs porin loss, efflux overexpression, and Ξ²-lactamases working together. Plus a surprising twist about Pseudomonas and ertapenem that challenges conventional teaching.
π Working through ROC-AUC from scratch, then poking at its blind spots β low prevalence, calibration, and finally Decision Curve Analysis. Mostly notes to myself on what I learned (and got confused by) along the way. π€π
Textbooks say LOOCV has the lowest bias but highest variance compared to 10 and 5-fold. Coded a K-Fold CV from scratch for learning to test that on simulated data ππ β and at n=1000 it holds up. At n=100? Not so much. π€
𧬠Back to basics! Learning non-polar amino acids, what zwitterions actually are, and dipping into the applied math β Rodrigues rotation and Lennard-Jones potential. Slowly building toward optimal phi/psi!
Exploring the CovR/S two-component system in Group A Strep 𧫠β from genome annotation with Bakta & BaktFold, to AlphaFold confidence metrics, and a first attempt at protein docking with Haddock3. Learning as we go! π