🦠 Made up my own memory tricks — Amp(G)atekeeper, Amp(D)umpster, Amp(R)epressor — to understand AmpC mechanics. Learnt Serratia has 2 recyclers (AmpD + AmiD2) making derepression harder, and E. coli lacks AmpR so AmpC stays silent! 🙌
Instead of flashcards, we Rube Goldberg’d this with Bioconductor! Analyzed 3,280 E. coli genomes from NCBI, detecting ESBL genes in 84.4% of samples. CTX-M-15 was most common. Helped us understand gene nomenclature and sequence analysis! 📊🔬
Wow, what a journey, and more to come! We learned how to perform simple RAG with an LLM and even ventured into LangChain territory. It wasn’t as scary as some people said! The documentation is fantastic. Best of all, we did it ALL in R with Reticulate, without leaving RStudio! Not only we can read IDSA Guidelines, we can use LLM to assist us with retrieving information!